·
Affinity
chromatography involves the covalent attachment of an immobilized biochemical
(called an affinity ligand) to a solid support.
·
When
the sample is passed through the column, only the solute that selectively binds
to the complementary ligand is retained.The other sample components elute with
out retention.
·
The
separation is well expressed by lock and key binding.
·
The
retained solute(s) can be eluted from the column by changing the mobile phase
conditions.
·
The
major advantage of this technique is its tremendous specificity, which permit
rapid isolation with a good yield in a single step.
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