Ascending chromatography is a chromatography technique
in which the mobile phase runs in an upward direction.
Descending chromatography is also one type of chromatography.
Descending chromatography is also one type of chromatography.
Paper chromatography and the thin layer chromatography
are the process in which the Ascending technique is used.
Principle:
In ascending, chromatography mobile phase moves in
upward direction opposite to the gravity by the capillary action, and the
separation of the analyte partition or adsorption principle is used.
Procedure
:
-To perform the Ascending chromatography Adsorption filter
paper/TLC plate, solvent as mobile phase, glass chamber with lid or beaker with
lead, fine capillary for spotting is required.
-Pour the required quantity of mobile phase in the chamber
or the Beaker.
TLC plate or the adsorption paper with required dimension
used as the stationary phase.
-Using
the syringe or glass capillary, spotting of the mixture of the analyte is a
place on the stationary phase at the marked place.
-Place
the stationary phase into the chamber or the beaker keeps the lid.
-The spot
of the mixture of analyte should not be immersed in the Mobile Phase.
The
mobile phase in ascending chromatography moves downward to the upward
direction.
-The
spot with a mixture of analyte travel through the mobile phase and separation
of the analyte is observed on the paper in the form of a spot.
-Reagent,
in case visual inspection of the spot is not possible (To visualize the spot)
Diagram:
 |
Types of Chromatography |
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