Definition
of Stationary Phase:
Stationary phase is one of the important components
of the chromatography where material is separated from the mixture of the
analyte.
-Stationary phase can be in solid, liquid or in gel
form, when mobile phase pass through the stationary phase, mixture of sample
also travel with the mobile phase and gets separated according to their
affinity towards the stationary phase.
-If component have more affinity toward the stationary
phase, it remains longer time in stationary phase and elute slowly. If
component have less affinity towards the stationary phase it quickly elutes or
separated out.
-When stationary phase is liquid or gas, it needs
the solid support for stationary phase. Stationary phase should remain constant
and inert in whole chromatography process.
-Depend on the chromatography, different types of
stationary phases are used.
Paper, gas, silica gel and agarose are several
examples of the stationary phase.
Here are some examples of chromatography and their
stationary phases used in process:
-Column chromatography: Silica gel, and alumina.
-Paper chromatography: Cellulose filter paper
-Thin-layer chromatography (TLC): Alumina, silica
gel, or similar material is coated on metal, plastic film or a glass plate, as
a thin layer.
-Gas chromatography (GC): A microscopic layer of
polymer on an inert solid support, inside the portion of metal or glass tubing.
-High-pressure liquid chromatography (HPLC): Silica
filled in the column.
-Ion exchange chromatography: Different type of cation
or anion resins
No comments:
Post a Comment