Elution in Chromatography

Elution, Eluate, Eluent is the different terminology  most widely used in chromatographic separation.
Here the Definition of the elution,
Elution is the process of extracting one material from another by washing with a solvent.


In chromatographic separation, the mobile phase is passed through the stationary phase after the application of the sample. For washing out a compound through a column suitable solvent must be used.


The solvent enter in the column is known as the Eluent and the solvent extract out from the column known as the Eluate. Eluent is only the solvent that works as carrier only. In HPLC different solvent acts as Eluent while in gas chromatography gas is acts as chromatography.
Eluate contains the mobile phase solvent plus the analyte material. It also contains the solute pass from the column.

Elution in Chromatography



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