HPLC is a form of liquid
chromatography used to separate compounds that are dissolved in solution. HPLC
instruments consist of a reservoir of mobile phase, a pump, an injector, a
separation column, and a detector.
Compounds are separated by injecting a sample mixture onto
the column. The different component in the mixture pass through the column at
differentiates due to differences in their partition behavior between the
mobile phase and the stationary phase. The mobile phase must be degassed to
eliminate the formation of air bubbles
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