Thin layer chromatography
- Thin layer chromatography is also known as TLC.
- TLC is one type of solid liquid chromatography.
- where mostly stationary phase is absorbent.usually it is finely ground silica or alumina particles.
- The absorbent is coated on the thin aluminum sheet.and mobile phase can be single solvent or mixture of solvents.
- TLC is quick and inexpensive technique it used to determined:
- Determine the number of component in a mixure.
- verify a substance's identify.
- monitor the progress of reactiochn. This technique is mainly used in active pharmaceutical ingredients plant to know the status of reaction.
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