Gel Permeation Chromatography (Type of Size exclusion Chromatography)

Gel permeation chromatography is one of the high performance liquid chromatography. It is the one type of the Size exclusion chromatography.

There is two type of Size exclusion chromatography

Gel permeation chromatography

Gel filtration chromatography

The main difference between the Gel permeation chromatography and the gel filtration chromatography is the Mobile Phase

Gel permeation Chromatography:

Where the solid stationary phase and mobile phase is non-polar.

Gel permeation Chromatography Principle

The Basic principle of the Size Exclusion Chromatography is Smaller the particle of the analyte is trapped into the Column bed. The column bed act as sieve which trapped the smaller molecule of the analyte easily, Hence Smaller the molecule of the analyte, it retains for more period time in SEC column temporarily

Large molecules are not trapped in the column bed. Large molecule passes around the bed and excluded from the column bed. Larger the molecule quickly pass through the column

Large molecules are eluted first from the column and then small molecules are eluted

In other terms, we can say that large molecule has less retention time while the small molecule has more retention time.