Application of Affinity Chromatography

Affinity chromatography most widely used for the separation of the specified biomolecule from the mixture. There is a number of applications of affinity chromatography.

Following are the different application of the affinity chromatography:

·        Antibodies:- antigen is immobilized and used to isolate and purify the appropriate antibody. The antibodies in a serum sample specific for a particular antigenic determinant can be isolated by the use of affinity chromatography.

·        Isolation of insulin receptor of liver cell.

·        Enzymes:- Substrate inhibitors can be identified.

·        Purify and concentrate a molecule from a mixture into a buffering solution

·        Reduce the amount of a molecule in a mixture

·        Discern what biological compounds bind to a particular molecule, such as drugs

In Clinical analysis & biotechnology

·        Microscale Molecular Diagnosis.

·        HPAC Used to determine the presence of specific components of biomolecular families in mixtures, including biological fluids & cell & tissue extract.

·        Compared with standard techniques for clinical determination & quantitation of biomolecules, analytical HPAC has a specificity  & the speed of operation that allow rapid detection  & simultaneous binding activity characterization of a whole set of molecules that are related functionally

·        Use of boronate, lectin, protein A or protein G & immunoaffinity support for the direct quantification of solutes.

·        Affinity based chiral separation & used AC for the study of a drug or hormone interaction will binding proteins

·        In therapeutic extracorporeal plasmapheresis & large scale fractionation of human plasma with special emphasis on an industrial rather than analytical scale.

·        In the study of glycobiology, application of glycol affinity chromatography

·        The interaction between recommendation ricin A chain & several triazine dyes were investigated to improve the purification of r-ricin A from E.coli fermentation.

·        Interaction of R- &-S ibuprofen with human serum albumin is studied.

Applications in pharmaceutical analysis

·        Isolation & detection of hygromycin B in a biological fluid

·        Column consists of lysozymes & α- lactalbumin immobilized on an N-hydroxy succinimide, agarose support & evaluated for the purificator on hygromycin In biological fluid

·        Disopyramide by capillary affinity electrokinetic chromatography with human α1acid glycoprotein(AGP) as chiral selector is studied

·        The interaction between recommendation ricin A chain & several triazine dyes were investigated to improve the purification of r-ricin A from E.coli fermentation.

·        The work is also carried out to compare & evaluate specificity & binding capacity of commercial & in house affinity columns  used in the sample preparation for analysis of growth-promoting drugs.