Types of Chromatography


Chromatography is a method used to separate the compound from the mixture. There are so many types of chromatography is used in Separation and identification of purity and impurity into the mixture. The selection of the chromatography technique depends on the types of the compound need to separate from the mixture and nature of the compound etc. Chromatography technique can be classified based on the stationary phase, principle, Mobile phase, operation.



Following are the different class of the chromatography :



Based on the stationary Phase
Based on the state of stationary phase
·        Solid chromatography
It also classified based on the mobile phase with the solid stationary phase.
o   Solid liquid chromatography
o   Solid gas chromatography
·        Liquid Chromatography
It also classified based on the mobile phase with the Liquid stationary phase.
o   Liquid Liquid chromatography
o    Liquid solid chromatography


Based on the shape of the stationary phase
·        Planar chromatography
There are different types of chromatography
o   Thin layer chromatography (TLC)
o   High performance thin layer chromatography (HPTLC)
o   Paper chromatography
·        Column Chromatography
Column chromatography further classified into the types of the stationary phase filled into the column
o   Packed column
o   Tubular chromatography

Based on the mobile phase

Based on the polarity

·        Reverse phase chromatography
·        Normal Phase Chromatography


Base on the form of mobile phase
·        Liquid chromatography
·        Gas chromatography
·        Supercritical fluid chromatography

Based on the principle of chromatography
Partition Chromatography
·        Liquid liquid chromatography
·        Liquid Gas chromatography

Adsorption chromatography
·        Column chromatography
·        Gas chromatography
·        TLC/HPTLC

Ion exchange chromatography
·        Anion Exchange  chromatography
·        Cation Exchange Chromatography

Affinity chromatography

Size exclusion chromatography
o   Gel permeation
o   Gel filtration chromatography

High performance liquid chromatography (HPLC)

Chiral chromatography

Ion pair chromatography

Base on the elution chromatography
·        Isocratic chromatography
·        Gradient chromatography

Based on the scale operation
·        Preparative chromatography
·        Analytical chromatography  

No comments:

Post a Comment