We can say that the High performance liquid chromatography is the
advanced of liquid chromatography. The main difference between the HPLC and the
liquid. Chromatography is High pressure
In liquid chromatography solvent pass through the force of gravity while
in HPLC the solvent pass under the high pressure
Other than that following are the difference :
Parameter
|
Convection column
chromatography
|
High
performance liquid Chromatography
|
particle size
|
Large (60-200µ)
|
Small (3-20µ)
|
Column length
|
0.5 -5m
|
5 -50 cm
|
Operating pressure
|
Low(<20 psi)
|
High(500-3000 psi)
|
Flow rate
|
Low to very low
|
Medium to high
|
Sample load
|
Low to medium (gm. or mg)
|
Low to very low (µg)
|
Resolving power
|
<500 theoretical plates per meter
|
>100000 plates per meter
|
Scale of operation
|
Preparative scale
|
Analytical and preparative scale
|
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