Resolution
means the separation of peak in a chromatogram.
In
Chromatographic language the resolution is
defined as quantitative measure of how well two elution peaks can be
differentiated in a chromatographic separation.
The
resolution depends on the selection of mobile phase, buffer composition, and
flow rate of mobile phase, sample nature and volume.
Calculation It is defined as the difference in
retention times between the two peaks, divided by the combined widths of the
elution peaks.
Optimum resolution shall be achieved in
specified time.
Resolution is affected by following parameters:
- Selectivity
- Efficiency
- Retention
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