Different types of chromatography technique available according the requirement and the principle chromatography technique used.
Chromatography have
vast area of the use in different industry.
Used in pharmaceutical
industry:
Detection of impurity
and unknown compound into the mixture during the process development and
analytical method development
Identify compound from
the mixture or the dosage form (Tablets, Capsule)
To perform assay ad
purity test of the product
To perform the organic
volatile impurities into the mixture. Gas chromatography is used for the
residual solvent test
For extraction purpose
Separation of the
chiral compound from the mixture is the most widely used of the chromatography
technique.
To purify the water
for manufacturing process Chromatography is used. Cation anion exchange
chromatography is used for Water purification system.
Check the quality of
the drinking water air
Used in phytochemistry:
Phytochemistry is the origin
of chromatography. chromatography was first developed by the Russian botanist Mikhail in
1903. In a column of calcium carbonate, a colorful separation of plant pigments
was produced by him. Chromatography has since developed into an invaluable
laboratory tool for the separation and identification of compounds.
Chromatography is used
to obtain the pure compound from the plant. Column chromatography is used to
isolation of the compound. TLC technique is used to identify the compound.
Used in food industry:
Now a day
chromatography is one of the preferable techniques for testing of food in
industry.
Chromatography
technique also used in food industry in various way
To identify the
additive into the food different types of the chromatography technique is used
One of the most common
use of the chromatography is to find out the food spoilage
Detection of the
nutrition of the food, Many types of amino acid, flavonoids, alkaloids,
protein, organic acid, Vitamins, mineral etc.
Also used in liquor
and alcohol testing industry to check the quality of the product
Used in polymer Industry:
Polymer Synthesis
To separate out the
polymer based on the size
Forensic testing
HPLC in figure print
and bioinformatics.
Gas chromatography is
used to determination the alcohol and drugs level in someone body
In Biotechnology:
Separation and the
purification of biological compound Separation of different compound of the
blood.
Purification of the
insulin is the example of using the chromatography
Plasma purification
Enzyme separation
Molecular biology
research and the DNA
Environmental Industry
Effluent monitoring
chromatography technique is used
In detection of small
amount of the contaminants and the oil droplets pollutant different
chromatography technique is used.
Most widely used in
environmental research laboratory for the detection of the pollutant
In Vaccination:
Ebolla immunization.
There are different
types of chromatography. According to the types of the chromatography there are
different use of the chromatography.
Example:
Paper chromatography
is used for the identify the basic compound from the mixture the first use of
the chromatography technique is separation of pigmentation from the plant
extraction.
HPLC (High performance
chromatography)
Ion exchange chromatography
Ion exchange chromatography are
most widely used in purification of the water for the pharmaceutical industry.
Ion exchange chromatography also used to monitor the fermentation process.
Size exclusion chromatography
As name suggested Size exclusion
chromatography is used for the separation and purification of molecule depend
on the size. To separate and purify the biological compound size exclusion
chromatography is used.
Size exclusion chromatography is
also used in separation of the molecular of the different size
Gas chromatography:
Gas chromatography is generally
used for detection of volatile compound or the compound which can convert into
their volatile form.
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